IBMX #13630
Product Information
Product Usage Information
IBMX is supplied as a lyophilized powder. For a 250 mM stock, reconstitute the 50 mg in 899.7 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1,000 µM for 15 min-4 hr.
Storage
Product Description
| Molecular Weight | 222.3 g/mol |
| Purity | >98% |
| Molecular Formula | C10H14N4O2 |
| CAS | 28822-58-4 |
| Solubility | Soluble in DMSO at 10mg/ml and EtOH at 5mg/ml. |
Background
3-isobutyl-1-methylxanthine (IBMX) is a nonselective inhibitor of cAMP and cGMP phosphodiesterases (PDEs). Research studies show that IBMX inhibits many members of the PDE family, with the exceptions of PDE8A, PDE8B, and PDE9 (1-4). Treatment of cells with IBMX promotes accumulation of cAMP and cGMP, which leads to activation of cyclic-nucleotide-regulated protein kinases (5,6). Additional research indicates that IBMX can promote neuronal progenitor cell maturation in vitro (7).
- Fawcett, L. et al. (2000) Proc Natl Acad Sci U S A 97, 3702-7.
- Fisher, D.A. et al. (1998) Biochem Biophys Res Commun 246, 570-7.
- Hayashi, M. et al. (1998) Biochem Biophys Res Commun 250, 751-6.
- Soderling, S.H. et al. (1998) J Biol Chem 273, 15553-8.
- Chanoit, G. et al. (2011) Cardiovasc Drugs Ther 25, 299-306.
- Zhang, R. et al. (2011) PLoS One 6, e20780.
- Lepski, G. et al. (2013) Front Cell Neurosci 7, 155.
Limited Uses
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