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Render Timestamp: 2024-12-20T10:51:57.322Z
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XML generation date: 2024-09-20 06:23:27.902
Product last modified at: 2024-08-23T13:30:07.637Z
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  1. Products /
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  3. Puromycin Dihydrochloride

Puromycin Dihydrochloride #40939

    Product Information

    Product Usage Information

    Puromycin Dihydrochloride is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 0.92 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

    Storage

    Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Molecular Weight 544.4 g/mol
    Purity >98%
    Molecular Formula C22H29N7O5 • 2HCl
    CAS 58-58-2
    Solubility Soluble in DMSO at 50 mg/mL or water at 50 mg/mL.

    Background

    Puromycin Dihydrochloride is hydrochloride salt that is part of the antibiotic puromycin, originally isolated from the bacterium Streptomyces alboniger. The clinical use of puromycin as an antibiotic has been limited as it is non-selective and highly toxic. Instead, puromycin has been used to study mechanisms of protein synthesis as it associates with growing polypeptide chains and causes premature chain termination (1). The structure of puromycin is analogous to the 3’ end of aminoacyl-tRNA, which allows puromycin to compete with aminoacyl-tRNA for binding at the A’ site of the peptidyl transferase center during protein translation. Puromycin binding results in premature chain release from the ribosome and a truncated, puromycylated polypeptide (2). As these puromycylated nascent polypeptide chains can be detected using anti-puromycin antibodies, incorporation of puromycin into newly synthesized proteins has been used to estimate the rate and localization of protein synthesis (3). The use of puromycin labeling to estimate protein synthesis may be limited by cell conditions (e.g., energy starvation); similar limits are seen in studies using puromycin to localize areas of protein synthesis (4,5).

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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