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Product last modified at: 2025-01-01T09:04:47.746Z
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PDP - Template Name: Monoclonal Antibody (Alexa Fluor Conjugate)
PDP - Template ID: *******c8ce56b
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

BATF3 (E3K5H) Rabbit mAb (Alexa Fluor® 647 Conjugate) #29501

Filter:
  • F
Flow Cytometry Image 1: BATF3 (E3K5H) Rabbit mAb (Alexa Fluor® 647 Conjugate)
Flow cytometric analysis of fixed and permeabilized human peripheral blood mononuclear cells using BATF3 (E3K5H) Rabbit mAb (Alexa Fluor® 647 Conjugate) (right) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (left), co-stained with CD11c (3.9) Mouse mAb (violetFluor™ 450 Conjugate).

To Purchase # 29501

Cat. # Size Qty. Price
29501S 100 µl
50 tests
$450

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated BATF3 (E3K5H) Rabbit mAb #16484.

Product Usage Information

Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

BATF3 (E3K5H) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total human BATF3 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BATF3 protein.

Background

BATF3 (Basic leucine zipper transcriptional factor ATF-like 3, p21SNFT) is a member of the basic leucine zipper family (1,2,5) which dimerizes with Jun proteins and represses activator protein-1 (AP-1) activity (1). BATF3 is required for development of conventional type 1 dendritic cells (cDC1s), which cross-present antigens in the context of MHC class I to naïve CD8+ T cells, priming them to become activated cytotoxic CD8+ T cells (2-4).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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