C/EBPβ (E2K1U) Rabbit mAb (Alexa Fluor® 594 Conjugate) #88314
- F
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
C/EBPβ (E2K1U) Rabbit mAb (Alexa Fluor® 594 Conjugate) recognizes endogenous levels of total C/EBPβ protein. This antibody does not cross-react with C/EBPα protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly67 of human C/EBPβ protein.
Background
CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions, and inflammatory response (1). Six members of the family have been characterized (C/EBPα, -β, -γ, -δ, -ε, and -ζ) and are distributed in a variety of tissues (1). There are two forms of C/EBPβ, the 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP) which may be products of alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPβ transcriptional activity (2). Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at serine 105 of rat C/EBPβ, a unique site only present in the rat sequence, seems essential for rat C/EBPβ activation (6).
- Lekstrom-Himes, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
- Calkhoven, C.F. et al. (2000) Genes Dev. 14, 1920-1932.
- Wegner, M. et al. (1992) Science 256, 370-373.
- Trautwein, C. et al. (1993) Nature 364, 544-547.
- Nakajima, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 2207-2211.
- Buck, M. et al. (1999) Mol. Cell 4, 1087-1092.
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