Render Target: STATIC
Render Timestamp: 2024-12-13T11:11:54.942Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-05-10 06:20:22.740
Product last modified at: 2024-12-02T16:15:09.092Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Caveolin-1 (D46G3) XP® Rabbit mAb (HRP Conjugate) #12506

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Hm Mk B Dg
    SENSITIVITY Endogenous
    MW (kDa) 21, 24
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 
    • B-Bovine 
    • Dg-Dog 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Caveolin-1 (D46G3) XP® Rabbit mAb #3267.
    MW (kDa) 21, 24

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

    Protocol

    Specificity / Sensitivity

    Caveolin-1 (D46G3) XP® Rabbit mAb (HRP Conjugate) detects endogenous levels of total caveolin-1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey, Bovine, Dog

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu20 of human caveolin-1 protein.

    Background

    The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homo-oligomers that interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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