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Product last modified at: 2025-01-01T09:06:37.382Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Caveolin-1 (D46G3) XP® Rabbit mAb (HRP Conjugate) #12506

Filter:
  • WB
Western Blotting Image 1: Caveolin-1 (D46G3) XP® Rabbit mAb (HRP Conjugate)
Western blot analysis of extracts from HeLa and NIH/3T3 cells using Caveolin-1 (D46G3) XP® Rabbit mAb (HRP Conjugate).

To Purchase # 12506

Cat. # Size Qty. Price Ships
12506S 100 µl
$450

Supporting Data

REACTIVITY H M R Hm Mk B Dg
SENSITIVITY Endogenous
MW (kDa) 21, 24
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Hm-Hamster 
  • Mk-Monkey 
  • B-Bovine 
  • Dg-Dog 

Product Information

Product Description

This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Caveolin-1 (D46G3) XP® Rabbit mAb #3267.
MW (kDa) 21, 24

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Protocol

Specificity / Sensitivity

Caveolin-1 (D46G3) XP® Rabbit mAb (HRP Conjugate) detects endogenous levels of total caveolin-1 protein.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey, Bovine, Dog

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu20 of human caveolin-1 protein.

Background

The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homo-oligomers that interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6).

Pathways

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For Research Use Only. Not For Use In Diagnostic Procedures.
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