Render Target: STATIC
Render Timestamp:
4/7/2025, 1:26:50 AM EDT
4/7/2025, 5:26:50 AM UTC
Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:17:56.921
Product last modified at: 2025-01-25T08:00:54.371Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

View Featured Offers >>
1% for the planet logo
PDP - Template Name: Monoclonal Antibody (Alexa Fluor Conjugate)
PDP - Template ID: *******c8ce56b
  1. Products /
  2. Antibody Conjugates /
  3. CD3ε (D7A6E™) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD3ε (D7A6E™) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #86936

Filter:
  • IHC
Immunohistochemistry Image 1: CD3ε (D7A6E™) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using CD3ε (D7A6E™) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and DAPI #4083 (blue).
1/3

To Purchase # 86936

Cat. # Size Qty. Price
86936S 100 µl
50 tests
$450

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated CD3ε (D7A6E™) XP® Rabbit mAb #85061.

Product Usage Information

Application Dilution
Immunohistochemistry (Paraffin) 1:100 - 1:400

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

CD3ε (D7A6E™) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total CD3ε protein. This antibody recognizes human CD3ε protein and is also reactive with mouse CD3ε; however, this antibody is not suggested for immunohistochemical analysis of mouse tissues.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu178 of human CD3ε protein.

Background

When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5).

Pathways

Explore pathways related to this product.


Database Links

UniProt ID: P07766


Entrez-Gene Id: 916


Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
XP is a registered trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.