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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD4 (RM4-5) Rat mAb (PE Conjugate) #26589

Filter:
  • F
Flow Cytometry Image 1: CD4 (RM4-5) Rat mAb (PE Conjugate)
Flow cytometric analysis of live mouse splenocytes using CD4 (RM4-5) Rat mAb (PE Conjugate) (solid line) compared to concentration-matched Rat Isotype Control (PE Conjugate) (dashed line).

To Purchase # 26589

Cat. # Size Qty. Price
26589S 100 µg
$76

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rat IgG2a kappa
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometric analysis in mouse cells.

Product Usage Information

For optimal flow cytometry results, we recommend 0.25 μg of antibody per test.

Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:80
Flow Cytometry (Live) 1:80

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

CD4 (RM4-5) Rat mAb (PE Conjugate) recognizes endogenous levels of total CD4 protein. This antibody detects an epitope within the extracellular domain.

Species Reactivity:

Mouse

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part, and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).
For Research Use Only. Not For Use In Diagnostic Procedures.
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