CD45 (D3F8Q) Rabbit mAb (Alexa Fluor^® 488 Conjugate) #59572

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Supporting Data

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

IHC-Immunohistochemistry
IF-Immunofluorescence

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 488 fluorescent dye. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated CD45 (D3F8Q) Rabbit mAb #70257.

Product Usage Information

Application	Dilution
Immunohistochemistry (Paraffin)	1:50
Immunofluorescence (Frozen)	1:50
Immunofluorescence (Immunocytochemistry)	1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

CD45 (D3F8Q) Rabbit mAb (Alexa Fluor^® 488 Conjugate) recognizes endogenous levels of total CD45 protein. This antibody is predicted to react with both the CD45.1 and CD45.2 alleles.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1258 of mouse CD45 protein.

Background

The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4).

Database Links

UniProt ID: P06800

Entrez-Gene Id: 19264

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CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 488 Conjugate) #59572