CD45R/B220 (RA3-6B2) Rat mAb (FITC Conjugate) #34399
- IF
- F
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rat IgG2a kappa |
Application Key:
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
Product Usage Information
For optimal flow cytometry results, we recommend 0.25 μg of antibody per test.
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:400 - 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 |
| Flow Cytometry (Live) | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The RA3-6B2 clone is raised against an isoform of CD45 known as CD45R/B220. CD45R/B220 is widely used as a marker for B cells, T cell subsets, and NK cell subsets in both human and mouse (5).
- Huntington, N.D. and Tarlinton, D.M. (2004) Immunol Lett 94, 167-74.
- Felberg, J. and Johnson, P. (2000) Biochem Biophys Res Commun 271, 292-8.
- Kashio, N. et al. (1998) J Biol Chem 273, 33856-63.
- Wang, Y. and Johnson, P. (2005) J Biol Chem 280, 14318-24.
- Hermiston, M.L. et al. (2003) Annu Rev Immunol 21, 107-37.
Limited Uses
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