CD45RA (HI100) Mouse mAb (violetFluor™ 450 Conjugate) #72177
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2b kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:20 |
| Flow Cytometry (Live) | 1:20 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Several isoforms of CD45 are generated through alternative splicing in a cell type-specific and activation state-specific manner. The HI100 antibody is widely used as a leukocyte marker for naïve and activated T cells. Naïve T cells are positive for CD45RA, where activated T cells are negative for CD45RA (5).
- Huntington, N.D. and Tarlinton, D.M. (2004) Immunol Lett 94, 167-74.
- Felberg, J. and Johnson, P. (2000) Biochem Biophys Res Commun 271, 292-8.
- Kashio, N. et al. (1998) J Biol Chem 273, 33856-63.
- Wang, Y. and Johnson, P. (2005) J Biol Chem 280, 14318-24.
- Cosenza-Nashat, M.A. et al. (2006) Brain Pathol 16, 256-65.
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