Render Target: STATIC
Render Timestamp: 2024-11-22T11:32:44.286Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:15:30.803
Product last modified at: 2024-09-20T07:04:38.159Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD8α (2.43) Rat mAb (violetFluor 450 Conjugate) #17083

Filter:
  • IF
  • F

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rat IgG2b
    Application Key:
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to violetFluor™ 450 and tested in-house for direct flow cytometric analysis in mouse cells.

    Product Usage Information

    For optimal flow cytometry results, we recommend 0.25 μg of antibody per test.

    Application Dilution
    Immunofluorescence (Frozen) 1:50 - 1:200
    Flow Cytometry (Fixed/Permeabilized) 1:80
    Flow Cytometry (Live) 1:80

    Storage

    Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    CD8α (2.43) Rat mAb (violetFluor™ 450 Conjugate) recognizes endogenous levels of total CD8α protein. This antibody detects an epitope within the extracellular domain.

    Species Reactivity:

    Mouse

    Source / Purification

    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

    Background

    Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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