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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Cleaved Caspase-9 (Asp315) (D8I9E) Rabbit mAb (PE Conjugate) #31245

Filter:
  • F
Flow Cytometry Image 1: Cleaved Caspase-9 (Asp315) (D8I9E) Rabbit mAb (PE Conjugate)
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with Etoposide #2200 (25 μM, overnight; green), using Cleaved Caspase-9 (Asp315) (D8I9E) Rabbit mAb (PE Conjugate).

To Purchase # 31245

Cat. # Size Qty. Price
31245S 100 µl
50 assays
$477

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved-Caspase-9 (Asp315) (D8I9E) Rabbit mAb #20750.

Product Usage Information

Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

Cleaved-Caspase-9 (Asp315) (D8I9E) Rabbit mAb (PE Conjugate) recognizes endogenous levels of caspase-9 protein only when cleaved at Asp315. Non-specific proteins that are induced by apoptosis under certain conditions may be detected.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp315 of human caspase-9 protein.

Background

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing, resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330, producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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