Render Target: STATIC
Render Timestamp: 2024-12-26T11:26:20.765Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-06 15:12:09.379
Product last modified at: 2024-12-17T19:02:36.739Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Cofilin (D3F9) XP® Rabbit mAb (HRP Conjugate) #8503

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  • WB

    Supporting Data

    REACTIVITY H M R Mk Dg
    SENSITIVITY Endogenous
    MW (kDa) 19
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Dg-Dog 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cofilin (D3F9) XP® Rabbit mAb #5175.
    MW (kDa) 19

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

    Protocol

    Specificity / Sensitivity

    Cofilin (D3F9) XP® Rabbit mAb (HRP Conjugate) detects endogenous levels of total cofilin1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, Dog

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human cofilin1 protein.

    Background

    Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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