CXCL1/CXCL2 (E5M6D) Rabbit mAb (Biotinylated) #45597
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 9 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
| MW (kDa) | 9 |
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
CXCL1/CXCL2 (E5M6D) Rabbit mAb (Biotinylated) recognizes endogenous levels of total CXCL1 and CXCL2 proteins. This antibody is more sensitive for detection of CXCL1 protein than CXCL2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with human CXCL1 recombinant protein.
Background
CXCL1 and CXCL2 (GRO-α and GRO-β) are two of the chemokines that act as ligands for the chemokine receptor CXCR2 (1). CXCR2 is expressed by several types of leukocytes and plays an important role in the recruitment of neutrophils to sites of inflammation (2). Although neutrophil recruitment is an important component of the innate immune system’s response to infection or injury, prolonged or excessive neutrophil recruitment can lead to inflammatory disease. Therefore, blocking CXCR2 has been pursued as a therapeutic approach for a variety of inflammatory diseases (3). In addition, CXCL1 and CXCL2 expression can be elevated in the tumor microenvironment, which contributes to tumor cell survival through enhanced angiogenesis and recruitment of immunosuppressive myeloid-derived suppressor cells (MDSCs) (4,5).
- Stadtmann, A. and Zarbock, A. (2012) Front Immunol 3, 263.
- Olson, T.S. and Ley, K. (2002) Am J Physiol Regul Integr Comp Physiol 283, R7-28.
- Cheng, Y. et al. (2019) Biochim Biophys Acta Rev Cancer 1871, 289-312.
- Haghnegahdar, H. et al. (2000) J Leukoc Biol 67, 53-62.
- Katoh, H. et al. (2013) Cancer Cell 24, 631-44.
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