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Render Timestamp: 2024-12-26T11:26:44.398Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-05-10 06:27:01.392
Product last modified at: 2024-12-17T19:05:11.893Z
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PDP - Template Name: Monoclonal Antibody (Alexa Fluor Conjugate)
PDP - Template ID: *******c8ce56b
  1. Products /
  2. Antibody Conjugates /
  3. EOMES (D8D1R) Rabbit mAb (Alexa Fluor® 488 Conjugate)
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

EOMES (D8D1R) Rabbit mAb (Alexa Fluor® 488 Conjugate) #96823

Filter:
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated EOMES (D8D1R) Rabbit mAb #81493.

    Product Usage Information

    Application Dilution
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:100

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    EOMES (D8D1R) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total EOMES protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro180 of human EOMES protein.

    Background

    The T-box family of transcription factors is named for their shared homology with the DNA binding domain of the mouse brachyury (T) gene product. Members of this family bind DNA and are capable of transcriptional activation. They also have evolutionarily conserved expression patterns and roles in embryonic development, primarily mesoderm development (1). EOMES, or Tbr2 (T-box brain 2), is a master regulator of mesoderm formation that is also essential for trophoblast formation, gastrulation, neurogenesis, and the differentiation of certain T cell subsets. Embryos from EOMES knockout mice die soon after implantation due to their inability to develop a trophoblast (2,3). Conditional neural knockout mice show defects in development of a specific population of neural progenitors known as intermediate-stage progenitor cells (IPCs) that give rise only to neurons (4,5). These cells are formed from the radial glia in the ventricular and sub-ventricular zones of the cortex. Expression of EOMES increases as cells develop from radial glia to IPCs and then decreases as IPCs progress to neurons. Recent evidence suggests that EOMES and IPCs may also play a role in neurogenesis in the adult hippocampal SGZ (5). EOMES is also a key transcription factor for memory T cells and for full effector differentiation of CD8+ T cells (6). Expression of EOMES is induced in CD8+ T cells following viral infection and bacterial infection where sufficient IL-12 has been produced to elicit acute host cell response (7).

    Database Links

    UniProt ID: O95936


    Entrez-Gene Id: 8320


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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