Fibronectin/FN1 (E5H6X) Rabbit mAb (Alexa Fluor^® 647 Conjugate) #72943

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 647 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Fibronectin/FN1 (E5H6X) Rabbit mAb #26836.

Product Usage Information

Application	Dilution
Immunofluorescence (Immunocytochemistry)	1:50 - 1:200

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

Fibronectin/FN1 (E5H6X) Rabbit mAb (Alexa Fluor^® 647 Conjugate) recognizes endogenous levels of total Fibronectin/FN1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a protein fragment corresponding to residues near the carboxy terminus of human Fibronectin/FN1 protein.

Background

Fibronectin is a multi-domain extracellular matrix (ECM) protein. The protein uses its different domains to bind distinct ECM components such as collagens, growth factors, and cell surface integrins to carry on its functions (1). Fibronectin has been implicated in many essential biological processes, including tissue repair, fibrosis, and tumor development (1,2). There are many fibronectin isoforms. Plasma fibronectin is synthesized by hepatocytes and exists as a compact, inactive conformation in the bloodstream. It is a major component of fibrin clots. Upon binding to integrins or other cell surface receptors, fibronectin switches to an extended conformation, and exposes its function domains to activated extracellular matrix assembly (3). Fibroblasts, endothelial cells, and many types of cancer cells have also been shown to synthesize cellular fibronectin isoforms. Among them, the EDA or EDB isoforms are particularly highly expressed during fibrosis and cancer development, and are potential diagnostic and therapeutic targets (4-6).

Database Links

UniProt ID: P02751

Entrez-Gene Id: 2335

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Fibronectin/FN1 (E5H6X) Rabbit mAb (Alexa Fluor® 647 Conjugate) #72943