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Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-04-03 10:40:09.641
Product last modified at: 2025-02-01T00:45:08.453Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

FoxO3a (75D8) Rabbit mAb (Biotinylated) #3938

Filter:
  • WB
Western Blotting Image 1: FoxO3a (75D8) Rabbit mAb (Biotinylated)
Western blot analysis of extracts from wild type (+/+) and FoxO3 knock-out (-/-) mouse brain, using FoxO3a (75D8) Rabbit mAb (upper) and FoxO1 (C29H4) Rabbit mAb #2880 (lower). (FoxO3 knock-out (-/-) mouse brain was kindly provided by Dr. Ron DePinho, Dana-Farber Cancer Institute, Boston, MA).

To Purchase # 3938

Cat. # Size Qty. Price
3938S 100 µl
$450

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 82 to 97
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The unconjugated FoxO3a (75D8) Rabbit mAb #2497 reacts with human, mouse and rat Fox03a protein. CST expects that FoxO3a (75D8) Rabbit mAb (Biotinylated) will also recognize Fox03a in these species.
MW (kDa) 82 to 97

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Protocol

Specificity / Sensitivity

FoxO3a (75D8) Rabbit mAb (Biotinylated) detects endogenous levels of total FoxO3a protein. The antibody does not cross-react with other members of the FoxO4 or FoxO1 families.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu50 of human FoxO3a.

Background

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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