Render Target: STATIC
Render Timestamp: 2024-11-21T14:13:16.845Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-04-05 20:48:18.695
Product last modified at: 2024-10-21T21:45:07.228Z
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PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c

GFP (5G4) Mouse mAb (Magnetic Bead Conjugate) #67090

Filter:
  • IP

    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Mouse IgG2b
    Application Key:
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is immobilized by the covalent reaction of formylbenzamide-modified antibody with hydrazide-activated magnetic bead. GFP (5G4) Mouse mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of GFP-tagged recombinant proteins. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated GFP (5G4) Mouse mAb #55494.

    Product Usage Information

    Application Dilution
    Immunoprecipitation 1:20

    Storage

    Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20. Store at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GFP (5G4) Mouse mAb (Magnetic Bead Conjugate) detects native GFP and YFP-tagged proteins exogenously expressed in cells, but does not detect denatured GFP. This antibody does not detect RFP-tagged proteins. GFP and YFP tags add approximately 27 kDa to the molecular weight of the fusion protein.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with native GFP.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage, and defines subcellular protein localization (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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