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Render Timestamp: 2024-12-26T11:22:38.501Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:23:02.909
Product last modified at: 2024-12-17T19:05:25.314Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Antibody Conjugates /
  3. GITR (E9O9H) Rabbit mAb (PE Conjugate)
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GITR (E9O9H) Rabbit mAb (PE Conjugate) #98032

Filter:
  • F

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometric analysis in mouse cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated GITR (E9O9H) Rabbit mAb #37472.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50
    Flow Cytometry (Live) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    GITR (E9O9H) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total mouse GITR protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val95 of mouse GITR protein.

    Background

    TNFRSF18, also known as glucocorticoid-induced tumor necrosis factor-receptor (TNFR)-related protein (GITR) and activation-inducible TNFR family receptor, encodes a type 1 membrane protein of the TNF-receptor superfamily (1). Three alternatively spliced transcript variants encoding distinct isoforms have been reported (2). GITR is an immune cell co-stimulatory receptor expressed constitutively at high levels on CD4+CD25+ T regulatory cells (Tregs), at low levels on naïve and memory T cells, and is induced upon T cell activation (3-5). Studies show GITR can also be induced on NK cells, macrophages, and DCs (3,4,6). Although GITR does not have intrinsic enzymatic activity, TNFSF18 (also known as GITRL) expressed on antigen presenting cells binds to GITR, resulting in recruitment of TNFR-associated factor family members and activation of the NF-κB pathway in T cells (7). GITR ligation has been shown to play a role in CD8+ T cell activation, cytotoxicity, and memory T cell survival (8-10). In the thymus, GITR is thought to play a key role in dominant immunological self-tolerance through thymic Treg differentiation and expansion (11). Of note, GITR ligation inhibits Treg suppressive function (12-13) and promotes effector T cell resistance to Treg suppression (14-15). Due to the combined effects on both Treg suppression and effector cell activation, GITR represents a unique opportunity for immunotherapeutic intervention in cancer (16).
    1. Nocentini, G. et al. (1997) Proc Natl Acad Sci U S A 94, 6216-21.
    2. Nocentini, G. et al. (2000) Cell Death Differ 7, 408-10.
    3. Shimizu, J. et al. (2002) Nat Immunol 3, 135-42.
    4. Nocentini, G. and Riccardi, C. (2009) Adv Exp Med Biol 647, 156-73.
    5. McHugh, R.S. et al. (2002) Immunity 16, 311-23.
    6. Hanabuchi, S. et al. (2006) Blood 107, 3617-23.
    7. Snell, L.M. et al. (2011) Immunol Rev 244, 197-217.
    8. Ronchetti, S. et al. (2007) J Immunol 179, 5916-26.
    9. Kim, I.K. et al. (2015) Nat Med 21, 1010-7.
    10. Snell, L.M. et al. (2012) J Immunol 188, 5915-23.
    11. Petrillo, M.G. et al. (2015) Autoimmun Rev 14, 117-26.
    12. Kanamaru, F. et al. (2004) J Immunol 172, 7306-14.
    13. Valzasina, B. et al. (2005) Blood 105, 2845-51.
    14. Stephens, G.L. et al. (2004) J Immunol 173, 5008-20.
    15. Nishikawa, H. et al. (2008) Cancer Res 68, 5948-54.
    16. Knee, D.A. et al. (2016) Eur J Cancer 67, 1-10.

    Database Links

    UniProt ID: O35714


    Entrez-Gene Id: 21936


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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