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Product last modified at: 2025-01-01T09:00:34.196Z
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PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

His-Tag (D3I1O) XP® Rabbit mAb (Magnetic Bead Conjugate) #96334

Filter:
  • IP
Immunoprecipitation Image 1: His-Tag (D3I1O) XP® Rabbit mAb (Magnetic Bead Conjugate)
Immunoprecipitation of an extract from 293T cells transfected with a construct expressing His-tagged full-length human Akt1. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) #8726, and lane 3 is His-Tag (D3I1O) XP® Rabbit mAb (Magnetic Bead Conjugate). Western blot analysis was performed using His-Tag (27E8) Mouse mAb #2366.

To Purchase # 96334

Cat. # Size Qty. Price
96334S 400 µl
$450

Supporting Data

REACTIVITY All
SENSITIVITY Transfected Only
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • All-All Species Expected 

Product Information

Product Description

This Cell Signaling Technology® antibody is immobilized by the covalent reaction of formylbenzamide-modified antibody with hydrazide-activated magnetic bead. His-Tag (D3I1O) XP® Rabbit mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of His-tagged recombinant proteins.

Product Usage Information

Application Dilution
Immunoprecipitation 1:20

Storage

Supplied in PBS Buffer (pH 7.2), 0.1% Tween 20. Store at 4°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

His-Tag (D3I1O) XP® Rabbit mAb (Magnetic Bead Conjugate) recognizes recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the 6xHis epitope tag.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

A variety of plasmids contain DNA that encodes an amino-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography (1).

As is the case with other protein tag systems (2), this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest (1).
For Research Use Only. Not For Use In Diagnostic Procedures.
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