Render Target: STATIC
Render Timestamp: 2024-12-13T11:15:34.382Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-27 20:01:29.368
Product last modified at: 2024-12-03T14:30:12.293Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Histone H3 (3H1) Rabbit mAb (HRP Conjugate) #5192

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  • WB

    Supporting Data

    REACTIVITY H M R Hm Mk Dm Z B Dg Pg
    SENSITIVITY Endogenous
    MW (kDa) 17
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 
    • Dm-D. melanogaster 
    • Z-Zebrafish 
    • B-Bovine 
    • Dg-Dog 
    • Pg-Pig 

    Product Information

    Product Description

    This Cell Signaling Technology (CST) antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (Histone H3 (3H1) Rabbit mAb #9717).
    MW (kDa) 17

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

    Protocol

    Specificity / Sensitivity

    Histone H3 (3H1) Rabbit mAb (HRP Conjugate) detects endogenous levels of total histone H3 protein. The antibody does not cross-react with other proteins.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey, D. melanogaster, Zebrafish, Bovine, Dog, Pig

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human histone H3.

    Background

    Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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