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Render Timestamp:
4/7/2025, 1:36:40 AM EDT
4/7/2025, 5:36:40 AM UTC
Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:20:07.668
Product last modified at: 2025-01-01T09:06:14.390Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

IFIT1 (D2X9Z) Rabbit mAb (PE Conjugate) #12983

Filter:
  • F
Flow Cytometry Image 1: IFIT1 (D2X9Z) Rabbit mAb (PE Conjugate)
Flow cytometric analysis of fixed/permeabilized human peripheral blood mononuclear cells, untreated (left column) or treated with Human Interferon-α1 (hIFN-α1) (10 ng/ml, 18 hr; right column), using IFIT1 (D2X9Z) Rabbit mAb (PE Conjugate) (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (bottom row).

To Purchase # 12983

Cat. # Size Qty. Price
12983S 100 µl
50 tests
$450

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology® antibody is conjugated to phycoerythrin (PE) under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated IFIT1 (D2X9Z) Rabbit mAb #14769.

Product Usage Information

Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

IFIT1 (D2X9Z) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total IFIT1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala194 of human IFIT1 protein.

Background

IFIT1 (interferon-induced protein with tetratricopeptide repeats 1) belongs to the IFIT family of proteins, which consists of four members in humans (IFIT1, IFIT2, IFIT3, and IFIT5) and three members in mice (IFIT1, IFIT2, and IFIT3) (1). IFIT1 expression is induced by type I interferons resulting from viral infection (2). IFIT1 is an antiviral protein that directly binds viral RNA that has a 5’ triphosphate group (PPP-RNA) (2). In humans, the viral PPP-RNA bound IFIT1 forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent replication (2). IFIT1 has also been shown to inhibit translation by binding to the eukaryotic initiation factor 3 (eIF3) (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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