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Render Timestamp: 2025-01-03T11:46:24.297Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-04-05 20:46:50.746
Product last modified at: 2024-05-30T07:19:49.305Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

KLRG1 (2F1) Hamster mAb (APC Conjugate) #26028

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Inquiry Info. # 26028

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    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Hamster (Syrian) IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to APC and tested in-house for direct flow cytometric analysis in mouse cells.

    Product Usage Information

    For optimal flow cytometry results, we recommend 0.06 μg of antibody per test.

    Application Dilution
    Flow Cytometry (Live) 1:300

    Storage

    Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    KLRG1 (2F1) Hamster mAb (APC Conjugate) recognizes endogenous levels of total KLRG1 protein. This antibody detects an epitope within the extracellular domain.

    Species Reactivity:

    Mouse

    Source / Purification

    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

    Background

    Killer cell lectin-like receptor G1 (KLRG1), also known as mast cell function associated antigen (MAFA), is an inhibitory type II transmembrane lectin-like receptor (1,2). This glycoprotein is expressed on a subset of natural killer (NK) and T cells (3,4). KLRG1 is considered a marker of T cell senescence (5,6). Studies have shown that KLRG1 is upregulated by MHC Class I molecule expression and these interactions can be mediated by lectin-like class I-specific Ly49 inhibitory receptors (1,3). KLRG1 triggering interferes with the activating TCR pathway and inhibits cytotoxicity (2). Specifically, KLRG1 binds to cadherin family ligands (E-, N-, and R-), inhibiting NK cell cytotoxicity (7). The effect that KLRG1 has on NK cell differentiation, maturation, homeostasis, and activation, make it an important target when studying innate immune regulation and chronic viral infections (8).
    The 2F1 antibody is widely used to detect KLRG1 on mouse cells (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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