MHC Class II (I-A/I-E) (M5/114.15.2) Rat mAb (APC-Cy7® Conjugate) #55413
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rat IgG2b kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
Product Usage Information
For optimal flow cytometry results, we recommend 0.5 μg of antibody per test. A slight precipitate may be present, but will not interfere with antibody performance. If precipitates are present, centrifuge the tube at 6,000xg for 10-30 sec. Draw off the supernatant and place into a light protective vial.
| Application | Dilution |
|---|---|
| Flow Cytometry (Live) | 1:40 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The M5/114.15.2 antibody reacts with mouse MHC class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes, but not from mice carrying the H-2s or H-2f haplotypes (4-7). The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes (8,9).
- Ting, J.P. and Trowsdale, J. (2002) Cell 109 Suppl, S21-33.
- Cresswell, P. (1994) Annu Rev Immunol 12, 259-93.
- Karp, D.R. et al. (1990) J Exp Med 171, 615-28.
- Staehli, F. et al. (2012) J Immunol 188, 3820-8.
- Scarlett, U.K. et al. (2012) J Exp Med 209, 495-506.
- Anderson, M.S. and Miller, J. (1992) Proc Natl Acad Sci U S A 89, 2282-6.
- Miyazaki, T. et al. (1996) Cell 84, 531-41.
- Parra, D. et al. (2012) J Leukoc Biol 91, 525-36.
- Chen, M. et al. (2011) J Immunol 187, 5684-92.
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