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MHC Class II (LGII-612.14) Mouse mAb (PE Conjugate)

MHC Class II (LGII-612.14) Mouse mAb (PE Conjugate) #84337

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Mouse IgG1

Application Key:

F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated MHC Class II (LGII-612.14) Mouse mAb #68258.

Product Usage Information

Application	Dilution
Flow Cytometry (Live)	1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

MHC Class II (LGII-612.14) Mouse mAb (PE Conjugate) exhibits strong reactivity with HLA-DRB and weak reactivity with HLA-DPB in cell lines transfected with constructs expressing Myc/DDK-tagged HLA-DRB and HLA-DPB, respectively. Reactivity is not observed with HLA-DMB, HLA-DOB, or HLA-DQB in cell lines transfected with constructs expressing Myc/DDK-tagged HLA-DMB, HLA-DOB, and HLA-DQB.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with cultured human B lymphoid cells treated with IFN-gamma.

Background

Major histocompatibility complex class II (MHC class II) molecules are heterodimeric, transmembrane glycoproteins expressed on the surface of antigen-presenting cells, such as macrophages, dendritic cells, and B cells. Expression can also be induced on other cell types through interferon-γ signaling (1). Prior to being displayed on the cell membrane, MHC class II molecules are loaded with exogenous peptide antigens approximately 15-24 amino acids in length that were derived from endocytosed extracellular proteins digested in the lysosome (2). Antigen-presentation through MHC class II is required for T cell activation during the immune response to extracellular pathogens (2). In humans, the MHC class II protein complex is encoded by the human leukocyte antigen gene complex (HLA). HLAs corresponding to MHC class II are HLA-DP, HLA-DM, HLA-DOA, HLA-DOB, HLA-DQ, and HLA-DR (3).

In the literature, this clone is reported to react with HLA-DR, HLA-DP, and HLA-DQ (4).

Database Links

UniProt ID: P01911 , P04440

Entrez-Gene Id: 3123 , 3115

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For Research Use Only. Not For Use In Diagnostic Procedures.

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