Render Target: STATIC
Render Timestamp: 2024-11-21T12:42:55.804Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:22:39.015
Product last modified at: 2024-11-11T13:15:44.586Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Myc-Tag (9B11) Mouse mAb (Pacific Blue Conjugate) #64584

Filter:
  • F

    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Mouse IgG2a kappa
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Myc-Tag (9B11) Mouse mAb #2276.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    Myc-Tag (9B11) Mouse mAb (Pacific Blue™ Conjugate) detects exogenously expressed proteins containing the Myc epitope tag. This antibody recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Myc-Tag (9B11) Mouse mAb (Pacific Blue™ Conjugate) detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. The antibody may cross-react with c-myc protein.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Pacific Blue is a trademark of Life Technologies Corporation.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.