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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

NK1.1/CD161 (PK136) Mouse mAb (redFluor 710 Conjugate) #19809

Filter:
  • F
Flow Cytometry Image 1: NK1.1/CD161 (PK136) Mouse mAb (redFluor™ 710 Conjugate)
Flow cytometric analysis of live mouse splenocytes using NK1.1 (CD161) (PK136) Mouse mAb (redFluor™ 710 Conjugate) (right) compared to concentration-matched Mouse Isotype Control (redFluor™ 710 Conjugate) (left), co-stained with CD3 (17A2) Rat mAb (FITC Conjugate) #86603.

To Purchase # 19809

Cat. # Size Qty. Price
19809S 100 µg
$238

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG2a kappa
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to redFluor™ 710 and tested in-house for direct flow cytometric analysis in mouse cells.

Product Usage Information

For optimal flow cytometry results, we recommend 0.125 μg of antibody per test.

Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:160
Flow Cytometry (Live) 1:160

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

NK1.1/CD161 (PK136) Mouse mAb (redFluor™ 710 Conjugate) recognizes endogenous levels of total NK1.1/CD161 protein. This antibody detects an epitope within the extracellular domain.

Species Reactivity:

Mouse

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

CD161/KLRB1 (Killer cell lectin-like receptor subfamily B member 1, also known as CLEC5B and NKR-P1A) is a type II transmembrane protein that is expressed on the majority of Natural Killer (NK) cells, NK T cells, and some T lymphocytes (1). CD161/KLRB1 is also expressed on Th17 cells, promotes their generation, and modulates their function (2). Engagement with its ligand lectin-like transcript 1 (LLT1) inhibits NK cell function, while LLT1 and CD161/KLRB1 interaction in the presence of a TCR signal enhances IFN-gamma production by T cells (3,4). There are several different CD161 isoforms in rodents and some function as activating receptors as well (5,6).

There is a family of Klrb1 genes in rodents (7). PK136 antibody recognizes a specific epitope on mouse KLRB1b/c, also referred to as NK1.1, and is commonly used for detection of mouse NK cells in certain mouse strains (CE, B6, NZB, C58, Ma/My, ST, SJL, FVB). However, the epitope is absent in other mouse strains (BALB/c, AKR, CBA, C3H, DBA, 129) (8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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