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Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (Alexa Fluor^® 647 Conjugate)

R Recombinant

Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (Alexa Fluor^® 647 Conjugate) #98523

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 647 fluorescent dye and tested in-house for direct immune fluorescent analysis in mouse tissue. This antibody is not expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb #20194. Species cross-reactivity for IF is mouse only.

Product Usage Information

Application	Dilution
Immunofluorescence (Frozen)	1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (Alexa Fluor^® 647 Conjugate) recognizes endogenous levels of tau protein when phosphorylated at Ser404. This antibody detects single phosphorylation at Ser404, dual phosphorylation at Ser400/Ser404 or Thr403/Ser404, and triple phosphorylation at Ser400/Thr403/Ser404. This antibody does not detect peptides with single phosphorylation at Ser400 or Thr403, and dual phosphorylation at Ser400/Thr403.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser400/Thr403/Ser404 of human Tau protein.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Database Links

UniProt ID: P10636-8

Entrez-Gene Id: 4137

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (Alexa Fluor® 647 Conjugate) #98523