Cell Signaling Technology Logo

Basket Updated

0

Items added

View Featured Offers >>
1% for the planet logo
  1. Products /
  2. Antibody Conjugates /
  3. Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix (Sepharose Bead Conjugate)
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix (Sepharose Bead Conjugate) #14005

Filter:
  • IP
Immunoprecipitation Image 1: Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix (Sepharose Bead Conjugate)
Immunoprecipitation of phospho-tyrosine proteins from A-431 cell extracts, untreated (-) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 5 min; +), using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423 (lanes 3 and 4), or Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix (Sepharose Bead Conjugate) (Lanes 5 and 6). Western blot analysis was performed using Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix (HRP Conjugate) #12695. Lanes 1 and 2 are 10% input.
1/1

To Purchase # 14005

Cat. # Size Qty. Price
14005S 400 µl
$431

Supporting Data

REACTIVITY All
SENSITIVITY Endogenous
MW (kDa) N/A
Source/Isotype Rabbit IgG
Application Key:
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • All-All Species Expected 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose beads.
Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of phospho-tyrosine containing proteins and peptides. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix #8954.
MW (kDa) N/A

Product Usage Information

Application Dilution
Immunoprecipitation 1:20

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Protocol

Specificity / Sensitivity

Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix (Sepharose Bead Conjugate) recognizes a broad range of tyrosine-phosphorylated proteins and peptides. This antibody does not cross-react with proteins or peptides containing phospho-Ser or phospho-Thr residues.

Species Reactivity:

All Species Expected

Source / Purification

MultiMab® rabbit monoclonal mix antibodies are prepared by combining individual rabbit monoclonal clones in optimized ratios for the approved applications. Each antibody in the mix is carefully selected based on motif recognition and performance in multiple assays. Each mix is engineered to yield the broadest possible coverage of the modification being studied while ensuring a high degree of specificity for the modification or motif.

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
MultiMab is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.