Render Target: STATIC
Render Timestamp: 2024-12-20T10:42:54.618Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:14:20.825
Product last modified at: 2024-11-08T12:30:11.637Z
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PDP - Template Name: Miscellaneous
PDP - Template ID: *******097325a
  1. Products /
  2. Antibody Conjugates /
  3. Protein A (HRP Conjugate)

Protein A (HRP Conjugate) #12291

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  • WB

    Product Information

    Product Usage Information

    This product should be used in place of the secondary in the western blotting protocol.

    Storage

    Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C.

    Protocol

    Product Description

    This Cell Signaling Technology product is useful for the detection of IgG. Conjugation of horseradish peroxidase (HRP) to protein A is obtained by cross-linking the amino groups on protein A with the carbohydrate groups on HRP.

    Specificity / Sensitivity


    Species Reactivity:

    All Species Expected

    Source / Purification

    Protein A (HRP Conjugate) is purified by gel filtration. The conjugate product is free from unconjugated protein A and HRP.

    Background

    Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and therefore can be used for detection and/or purification of antibodies. Due to its vast array of applications, protein A is the method of choice for many preparative and analytical immunological assays (1).

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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