Render Target: STATIC
Render Timestamp: 2024-12-30T10:40:23.954Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-07-25 12:37:16.102
Product last modified at: 2024-12-05T20:15:09.199Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

PU.1 (E8I8L) Mouse mAb (PE Conjugate) #20251

Filter:
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1 kappa
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to phycoerythrin (PE) under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated PU.1 (E8I8L) Mouse mAb #89136.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    PU.1 (E8I8L) Mouse mAb (PE Conjugate) recognizes endogenous levels of total PU.1 protein. This antibody does not cross-react with other Ets family members.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp26 of human PU.1 protein.

    Background

    PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box (1). PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells, including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells, and megakaryocytes (1,2). The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regulation of differentiation versus stem cell proliferation (3,4). In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by CK2 promotes binding to IRF-4 and synergistic activation through the immunoglobulin κ 3' enhancer (5). Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resulting in increased PU.1 activity and activation of the anti-apoptotic gene MCL-1 (6). GATA1 binding blocks PU.1 activity during erythroid cell development (7). Overexpression of PU.1 resulting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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