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PU.1 (F2D5A) Mouse mAb (PE Conjugate) #57800

Filter:
  • F
Flow Cytometry Image 1: PU.1 (F2D5A) Mouse mAb (PE Conjugate)
Flow cytometric analysis of fixed/permeabilized Neuro-2a cells (blue, negative) and RAW 264.7 cells (green, positive) using PU.1 (F2D5A) Mouse mAb (PE Conjugate) (solid lines) or concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control (PE Conjugate) #6899 (dashed lines).

To Purchase # 57800

Cat. # Size Qty. Price
57800S 100 µl
50 tests
$450

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG1 kappa
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology® antibody is conjugated to phycoerythrin (PE) under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated PU.1 (F2D5A) Mouse mAb #57906.

Product Usage Information

Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

PU.1 (F2D5A) Mouse mAb (PE Conjugate) recognizes endogenous levels of total PU.1 protein.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu27 of mouse PU.1 protein.

Background

PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box (1). PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells, including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells, and megakaryocytes (1,2). The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regulation of differentiation versus stem cell proliferation (3,4). In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by CK2 promotes binding to IRF-4 and synergistic activation through the immunoglobulin κ 3' enhancer (5). Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resulting in increased PU.1 activity and activation of the anti-apoptotic gene MCL-1 (6). GATA1 binding blocks PU.1 activity during erythroid cell development (7). Overexpression of PU.1 resulting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia (8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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