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S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor^® 700 Conjugate)

S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor^® 700 Conjugate) #56196

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Supporting Data

REACTIVITY	H M R Mk Dm
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Mouse IgG1

Application Key:

IF-Immunofluorescence
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey
Dm-D. melanogaster

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 700 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric in human and mouse cells and immunofluorescent analysis in human cells and mouse tissue. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated S6 Ribosomal Protein (54D2) Mouse mAb #2317.

Product Usage Information

Application	Dilution
Immunofluorescence (Frozen)	1:100
Immunofluorescence (Immunocytochemistry)	1:50 - 1:100
Flow Cytometry (Fixed/Permeabilized)	1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor^® 700 Conjugate) detects endogenous levels of total S6 ribosomal protein independent of phosphorylation.

Species Reactivity:

Human, Mouse, Rat, Monkey, D. melanogaster

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fusion protein corresponding to full-length human S6 ribosomal protein. The epitope corresponds to a region surrounding Asp20 of human S6 ribosomal protein.

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).

Database Links

UniProt ID: P62753

Entrez-Gene Id: 6194

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For Research Use Only. Not For Use In Diagnostic Procedures.

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XP is a registered trademark of Cell Signaling Technology, Inc.

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S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 700 Conjugate) #56196