Render Target: STATIC
Render Timestamp: 2024-12-20T10:54:33.361Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-10-10 15:16:23.685
Product last modified at: 2024-12-17T18:48:58.991Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Antibody Conjugates /
  3. S6 Ribosomal Protein (54D2) Mouse mAb (HRP Conjugate)

S6 Ribosomal Protein (54D2) Mouse mAb (HRP Conjugate) #14662

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk Dm
    SENSITIVITY Endogenous
    MW (kDa) 32
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Dm-D. melanogaster 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated S6 Ribosomal Protein (54D2) Mouse mAb #2317.
    MW (kDa) 32

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

    Protocol

    Specificity / Sensitivity

    S6 Ribosomal Protein (54D2) Mouse mAb (HRP Conjugate) detects endogenous levels of total S6 ribosomal protein independent of phosphorylation.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, D. melanogaster

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant fusion protein corresponding to full-length human S6 ribosomal protein.

    Background

    One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).

    Database Links

    UniProt ID: P62753


    Entrez-Gene Id: 6194


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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