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XML generation date: 2024-12-24 23:02:08.051
Product last modified at: 2024-12-25T09:00:50.825Z
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Streptavidin (Alexa Fluor® 647 Conjugate) #55871

    Product Information

    Product Description

    Streptavidin is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and is useful for detecting biotinylated proteins (1,2).

    Storage

    Store at 4°C. This product is stable for six months after receipt and is supplied in 10 mM KPi, 150 mM NaCl, and 5 mM sodium azide (pH 7.2).

    Protect from light. Avoid repeated freeze/thaw cycles.

    Specificity / Sensitivity

    Streptavidin has a remarkably high affinity for its natural ligand, biotin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).

    Source / Purification

    Streptavidin is a protein that is derived from the bacteria Streptomyces avidinii. Streptavidin (Alexa Fluor® 647 Conjugate) is purified by size exclusion chromatography.

    Background

    Streptavidin is a 53 kDa homotetramer isolated from Streptomyces avidinii for use in isolation and detection bioassays (3). Each streptavidin subunit forms high affinity non-covalent bonds with the vitamin biotin. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate and detect biotinylated proteins (1,2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for Immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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