Render Target: STATIC
Render Timestamp: 2024-12-13T12:20:28.975Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-12-05 11:37:13.138
Product last modified at: 2024-11-25T22:00:09.779Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SV40 Large T Antigen (D1E9E) Rabbit mAb (Sepharose® Bead Conjugate) #45159

Filter:
  • IP

    Supporting Data

    REACTIVITY All
    SENSITIVITY Endogenous
    MW (kDa) 90
    Source/Isotype Rabbit IgG
    Application Key:
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads.
    SV40 Large T Antigen (D1E9E) Rabbit mAb (Sepharose® Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same cross-species reactivity as the unconjugated SV40 Large T Antigen (D1E9E) Rabbit mAb #15729.
    MW (kDa) 90

    Product Usage Information

    Application Dilution
    Immunoprecipitation 1:20

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SV40 Large T Antigen (D1E9E) Rabbit mAb (Sepharose® Bead Conjugate) recognizes transgenic levels of total SV40 Large T Antigen protein.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys315 of SV40 Large T Antigen protein.

    Background

    Simian Virus 40 (SV40) is a small double-stranded, circular DNA tumor virus encoding three tumor antigens: large T, Small T, and 17KT. These antigens contribute to cellular transformation, an area of research where they are utilized extensively. Transformation of cells by SV40 large T antigen is mediated by its binding to the tumor suppressor proteins RB and p53 in the nucleus. Additional binding partners include p300, CBP, Cul7, IRS1, Bub1, Nbs1, and Fbw7, which also contribute to transformation (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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