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Tau (Tau46) Mouse mAb (Biotinylated)

Tau (Tau46) Mouse mAb (Biotinylated) #96767

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	50-80
Source/Isotype	Mouse IgG1

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Tau (Tau46) Mouse mAb #4019.

MW (kDa)

50-80

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Tau (Tau46) Mouse mAb (Biotinylated) detects endogenous levels of total tau protein and also cross-reacts with MAP2 at 280 kDa. Tau (Tau46) Mouse mAb (Biotinylated) is predicted to detect all six isoforms of tau based on the amino acid sequence.

Species Reactivity:

Human, Mouse, Rat

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Bovine

Source / Purification

Monoclonal antibody is produced by immunizing animals with native bovine tau and the epitope maps to the carboxy-terminus of the protein.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Database Links

UniProt ID: P10636-8

Entrez-Gene Id: 4137

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For Research Use Only. Not For Use In Diagnostic Procedures.

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