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Product last modified at: 2025-03-24T12:00:13.652Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Ubiquitin (P4D1) Mouse mAb (HRP Conjugate) #14049

Filter:
  • WB
Western Blotting Image 1: Ubiquitin (P4D1) Mouse mAb (HRP Conjugate)
Western blot analysis of HeLa cells, untreated (-) or treated with the 26S proteasome inhibitor MG-132 #2194 (50 µM, 90 min; +), using Ubiquitin (P4D1) Mouse mAb (HRP Conjugate).

To Purchase # 14049

Cat. # Size Qty. Price
14049S 100 µl
$450

Supporting Data

REACTIVITY All
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • All-All Species Expected 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ubiquitin (P4D1) Mouse mAb #3936.

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Protocol

Specificity / Sensitivity

Ubiquitin (P4D1) Mouse mAb (HRP Conjugate) detects ubiquitin, polyubiquitin, and ubiquitinated proteins. This antibody may cross-react with recombinant NEDD8.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with 1-76 full-length bovine ubiquitin.

Background

Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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