Render Target: STATIC
Render Timestamp: 2024-11-15T11:08:27.582Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-05-10 06:27:11.327
Product last modified at: 2024-07-08T14:45:09.560Z
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PDP - Template Name: Monoclonal Antibody (Alexa Fluor Conjugate)
PDP - Template ID: *******c8ce56b
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

UCP1 (E9Z2V) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #54262

Filter:
  • IF

    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct immunofluorescent analysis in mouse tissue. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated UCP1 (E9Z2V) XP® Rabbit mAb #72298.

    Product Usage Information

    Application Dilution
    Immunofluorescence (Frozen) 1:50 - 1:200

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    UCP1 (E9Z2V) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total UCP1 protein. This antibody does not cross-react with UCP2 or UCP3 protein.

    Species Reactivity:

    Mouse, Rat

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro152 of human UCP1 protein.

    Background

    Uncoupling protein 1 (UCP1) is a mitochondrial inner membrane transport protein that is primarily expressed in brown adipose tissue (BAT). UCP1 dissipates the pH gradient resulting from oxidative phosphorylation, which uncouples ATP synthesis from oxidative phosphorylation and leads to the release of heat energy. As a result, UCP1 plays an important role in thermogenesis (reviewed in 1). Research studies indicate that subcutaneous white adipose depots in mice contain beige adipocytes that express low levels of UCP1 protein (2). Additional studies show possible differences in thermogenesis in individuals carrying specific polymorphisms in the corresponding UCP1 gene (3). Related studies link UCP1 to the possible development of obesity and type 2 diabetes (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.