Whitlow/218 Linker (E3U7Q) Rabbit mAb (Biotinylated) #32523

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Supporting Data

REACTIVITY	All
SENSITIVITY	Transfected Only
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

F-Flow Cytometry

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Whitlow/218 Linker (E3U7Q) Rabbit mAb #57710.

Product Usage Information

Application	Dilution
Flow Cytometry (Live)	1:50

Storage

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Whitlow/218 Linker (E3U7Q) Rabbit mAb (Biotinylated) recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow/218 linker.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow/218 linker peptide.

Background

The Whitlow/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1).

Whitlow, M. et al. (1993) Protein Eng 6, 989-95.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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