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XML generation date: 2024-09-20 06:22:39.518
Product last modified at: 2024-09-16T17:30:09.387Z
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PDP - Template Name: Cellular Dyes
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7-AAD Cell Staining Solution #47501

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    Product Information

    Product Usage Information

    As a last step for flow cytometric assays, add 10 μl of 7-AAD Cell Staining Solution to 100 μl of sample. Incubate in the dark for 10 min at room temperature before analyzing cells on flow cytometer.

    Storage

    Store at 4ºC protected from light.

    Fluorescent Properties

    • Free dye excitation maximum = 503 nm
    • Free dye emission maximum = 675 nm
    • DNA complex excitation maximum = 543 nm
    • DNA complex emission maximum = 655 nm

    Product Description

    7-AAD Cell Staining Solution is supplied in an aqueous buffered solution containing less than 0.09% sodium azide. It can be used to stain apoptotic cells.

    Background

    7-Aminoactinomycin D (7-AAD) is a fluorescent DNA dye that binds poly(dG-dC) regions with an excitation maxima at 543 nm when bound to DNA (1). 7-AAD can be used as dead cell discriminating dye due to its exclusion from live cells with intact membranes (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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