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Product last modified at: 2024-09-13T22:45:07.181Z
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PDP - Template Name: Buffer Kit
PDP - Template ID: *******aace572

Cell Fractionation Kit (Eco-friendly detergent) #42193

    Product Information

    Storage

    Store at -20ºC. Upon receipt, Protease Inhibitor Cocktail (100X) #5871 should be stored at 4ºC. All other components should be stored at -20ºC.

    Protocol

    Product Description

    The Cell Fractionation Kit is designed to provide a fast and efficient way of separating cultured cells into three distinct fractions: cytoplasmic, membrane/organelle, and nuclear/cytoskeletal. These fractions can then be analyzed by SDS-PAGE and western blotting. The kit includes enough buffer for 20 assays.

    This kit uses ECOSURF SA-9, a non-toxic, biodegradable, non-ionic alkylpolyglucoside-based surfactant, in place of non-ionic surfactant Triton X-100 in the CST Cell Fractionation Kit #9038. Use of Triton X-100 use is being phased out of many laboratory settings due to concerns about toxicity and environmental impact. ECOSURF SA-9 is derived from seed oil and follows the "Design for Degradation" principle of Green Chemistry, thus providing a more environmentally safe option.ECOSURF SA-9 has comparable surfactant properties to Triton X-100, it does not react with charged molecules (proteins, nucleic acids), and it is stable over a wide pH range, making it a suitable replacement for many biological applications including cell fractionation. This kit performs equally as well as the original Triton-based kit.

    Background

    Cellular fractionation allows for the extraction of cellular proteins into distinct compartments. This is achieved by the use of detergents that take advantage of the inherent qualities and composition of different cellular membranes (1). Cellular fractionation has been important for defining the localization of many proteins, observing the translocation of proteins, and determining protein-protein complexes, such as cytoskeletal-associated proteins (2,3). Thus, detergent-based cellular fractionation separates cellular components with greater ease and speed compared to a more laborious density centrifugation method (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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