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XML generation date: 2024-10-22 19:26:09.174
Product last modified at: 2024-11-15T22:00:09.497Z
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PDP - Template Name: Buffer
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DTT (Dithiothreitol) #7016

    Product Information

    Product Usage Information

    DTT is supplied as a lyophilized powder. To prepare a 1 M stock solution, reconstitute the 192.8 mg supplied in the product tube in 1.12 ml ddH2O. The dissolved DTT will increase the final volume to approximately 1.25 ml.

    Storage

    Store lyophilized at 4ºC, desiccated. In lyophilized form, the chemical is stable for 12 months. Once in solution, store at -20ºC and use within 12 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Dithiothreitol (DTT) from Cell Signaling Technology is offered in a convenient 192.8 mg lyophilized format, allowing for preparation of a fresh stock solution. This DTT reagent contains no detectable DNase or RNase activity and is suitable for use in molecular biology or protein biochemistry applications that require reduction of protein disulfide bonds.

    SDS-PAGE sample buffers are routinely supplemented with 10-50 mM DTT to cleave protein disulfide bonds. Lower concentrations of DTT are routinely used to stabilize enzymes or other proteins that posses free sulfhydryl groups, which is useful in chromatin immunoprecipitation (ChIP) assays.
    MW (kDa) 154.25 g/mol
    Purity >99%
    Molecular Formula C4H10O2S2
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.