Render Target: STATIC
Render Timestamp: 2025-01-21T11:10:38.147Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-11-05 01:05:08.655
Product last modified at: 2025-01-16T19:45:10.108Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Buffer
PDP - Template ID: *******ceb613c

SignalStain® EDTA Unmasking Solution (10X) #14747

To Purchase # 14747

Cat. # Size Qty. Price Ships
14747P 25 ml
$27
14747S 125 ml
$107

Product Information

Product Usage Information

Dilute to a 1X Unmasking Solution using ddH2O just before use. Bring slides to a boil in 1X SignalStain® EDTA Unmasking Solution. Follow with 15 min at a sub-boiling temperature. No cooling is necessary.

Antigen unmasking protocols utilizing a water bath are not recommended. While antigen unmasking performed with a microwave is preferred, the staining of particular tissues or antigen targets may require the use of a pressure cooker.

Storage

Store 10X buffer at 4°C. At 10X, this buffer is stable for 12 months. Always prepare fresh 1X solutions daily.

Product Description

SignalStain® EDTA Unmasking Solution (10X) is used for antigen unmasking of formalin fixed, paraffin-embedded tissue sections or cell pellets in immunohistochemical assays (IHC-P). Cell Signaling Technology recommends the optimal unmasking reagent for each IHC-P approved antibody. Please consult the primary antibody datasheet to determine if this solution is recommended for your specific product.

Background

Proteins within fixed tissue sections contain chemical cross-links that may prevent antibody access or mask antigen targets in immunohistochemical assays. This limited access may vary among different tissue or antigens. Heat-induced antigen unmasking improves accessibility of antibodies to tissues or antigens (1).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.