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ER-Tracker Green (BODIPY FL Glibenclamide) #8787

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Immunofluorescence Image 1: ER-Tracker Green (BODIPY  FL Glibenclamide)
Confocal immunofluorescent analysis of live HeLa cells using ER-Tracker Green (BODIPY FL Glibenclamide) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

To Purchase # 8787

Cat. # Size Qty. Price
8787S 100 µg
$406
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Product Information

Product Usage Information

ER-Tracker Green (BODIPY FL Glibenclamide) is supplied as a single 100 μg lyophilized vial. To make a 1 mM stock solution, reconstitute the solid in 128 μl of high quality DMSO. Dilute 1:500 directly into normal growth media for a working concentration of 2 μM and analyze immediately. Cells must be imaged live; DO NOT FIX!

To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging.

Storage

Store lyophilized solid or solution at –20°C dessicated and protected from light. Once reconstituted in DMSO, the solution should be aliquoted to avoid freeze-thaw cycles. If stored properly, this reagent is stable for 6 months.

Product Description

ER-Tracker Green (BODIPY FL Glibenclamide) is recommended for live cell imaging only; fixation with aldehydes or alcohols will inhibit staining. Excitation: 504 nm, Emission: 511 nm, Molecular Weight: 783.10 g/mol

Background

ER-Tracker Green (BODIPY FL Glibenclamide) is a cell-permeant, live cell stain that is highly selective for the endoplasmic reticulum (ER). This stain consists of the green-fluorescent BODIPY FL dye and glibenclamide. Glibenclamide (glyburide) binds to sulphonylurea receptors, the regulatory subunit of ATP-sensitive K+ channels prominently expressed in the ER. The pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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