Render Target: STATIC
Render Timestamp: 2024-11-20T10:49:18.332Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:20:13.965
Product last modified at: 2024-10-25T16:00:11.713Z
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PDP - Template Name: Buffer Kit
PDP - Template ID: *******aace572

FoxP3/Transcription Factor Fixation/Permeabilization Kit #43481

    Product Information

    Reagents Not Included

    1. Red blood cell lysis buffer 2. Unconjugated or conjugated primary antibody 3. Fluorochrome-conjugated secondary antibody (if applicable) NOTE: When using any primary or secondary antibody for the first time, titrate the antibody to determine which dilution allows for the strongest specific signal with the least background for your sample. 4. Cellular dyes (e.g., live-dead discrimination, DNA dyes, etc.), optional

    Storage

    Store at 4ºC. All components in this kit are stable for at least 6 months when stored at 4ºC.

    Protocol

    Product Description

    This kit provides the reagents needed to support antibody-based detection of FoxP3 and other transcription factors by flow cytometry. Optimally formulated fixation and permeabilization buffers halt biological activity and enable antibody access to intracellular targets. Antibody dilution, incubation, and wash steps are performed with the included FoxP3/Transcription Factor Permeabilization Buffer, eliminating the need for separate reagents.

    Note: Precipitation may occur in some kit components. The presence of precipitate does not affect the performance of the reagent.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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