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Human Fc Receptor Blocking Solution

Human Fc Receptor Blocking Solution #58948

Name: Human Fc Receptor Blocking Solution
Price: 176.00 USD
Availability: InStock

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Product Information

Product Usage Information

Prior to immunostaining, treat cells with Human Fc Receptor Blocking Solution for 10 min at room temperature, using 2.5 μg/1 million cells in approximately 100 μL Antibody Dilution Buffer (may be purchased as ready-to-use Flow Cytometry Antibody Dilution Buffer #13616 or prepared as a solution of 0.5% Bovine Serum Albumin in 1X Phosphate Buffered Saline). No wash step is needed before incubating cells with primary antibodies.

Storage

Supplied in PBS solution (pH 7.2). Stable at 4°C for 1 year after receipt. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

Human Fc receptors cross-react with certain subclasses of IgG from species such as mouse, rabbit, and rat. When cells of interest express high levels of Fc receptor protein (for example, macrophage/monocyte lineages), pre-incubating live cells with Human Fc Receptor Blocking Solution prior to immunostaining will reduce this cross-reactivity.

Specificity / Sensitivity

Species Reactivity:

Human

Source / Purification

Modified human IgG1 was expressed in 293 cells.

Background

Human Fc Receptor Blocking Solution contains a modified form of human IgG1 that blocks human Fc receptors.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.

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