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Render Timestamp: 2024-12-26T10:58:06.381Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:19:40.603
Product last modified at: 2024-12-18T22:15:09.055Z
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PDP - Template Name: Buffer
PDP - Template ID: *******ceb613c

Kinase Buffer (10X) #9802

    Product Information

    Product Usage Information

    1. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. For longer periods of time, buffer should be stored at –20°C. Aliquotting of 10X buffer is recommended if many small experiments are to be performed.

    2. Thaw 10X buffer at 24-30°C, mixing end-over-end.

    3. Dilute the 10X buffer to the desired concentration using ddH2O.

    Solutions and Reagents

    1X Kinase Buffer25 mM Tris-HCl (pH 7.5), 5 mM beta-glycerophosphate, 2 mM dithiothreitol (DTT), 0.1 mM Na3VO4, 10 mM MgCl2.Note: Kinase Buffer must be supplemented with ATP (#9804).

    Storage

    Store at -20C. For short term storage (1-2 weeks), Kinase Buffer can be stored at 4°C.

    Product Description

    Kinase Buffer can be used to assay protein kinase activity.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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