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Lambda Protein Phosphatase Kit #89726

Lambda Protein Phosphatase Kit: Image 1
Western blot analysis of extracts from serum-starved MCF7 cells, untreated (-) or treated (+) with combinations of the following treatments as indicated: human IGF-1 (hIGF-1; 100 ng/mL, 10 min) and lambda protein phosphatase, using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).

To Purchase # 89726

Cat. # Size Qty. Price Ships
89726S 1 Kit
$765
  • Product Includes
Product IncludesQuantity (with Count)Storage Temp
Lambda Protein Phosphatase #562061 x 250 µl-80°C
Buffer for Protein MetalloPhosphatases (PMP) #908921 x 1 ml-20°C
MnCl2 for PMP #235171 x 1 ml-20°C

Product Information

Product Usage Information

1. Add protein sample and H2O (if necessary) to make a total volume of 40 μL.

2. Add 5 μL of 10X Buffer for Protein MetalloPhosphatases (PMP) and 5 μL of 10 mM MnCl2 for PMP to make a total reaction volume of 50 μL.

3. Add 1 μL of Lambda Protein Phosphatase.

4. Incubate at 30°C for 30 min.

Note: The optimal incubation times and enzyme concentrations must be determined empirically for each individual substrate.

Storage

All components in this kit are stable for 24 months when stored at the recommended temperature. Aliquot to avoid repeated freeze/thaw cycles.

Product Description

The Lambda Protein Phosphatase Kit provides reagents that, when combined, have activity toward phosphorylated serine, threonine, and tyrosine residues. Lambda Protein Phosphatase is the 221 amino acid product of the ORF221 open reading frame on bacteriophage lambda. It is also an Mn2+ dependent protein phosphatase that has the ability to release phosphate groups from phosphorylated serine, threonine, and tyrosine residues in proteins. Lambda Protein Phosphatase has a concentration of 400,000 units per mL.
Activity 800,000 units per mg

Unit Definition: One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50 mM) in 1 min at 30°C in a total reaction volume of 50 µL.

Specificity / Sensitivity

The Lambda Protein Phosphatase Kit is active toward phosphorylated serine, threonine, and tyrosine residues.

Source / Purification

The Lambda Protein Phosphatase component is produced in a strain of E. coli that carries the bacteriophage lambda ORF221 open reading frame. The product is isolated using the T7 expression system.
For Research Use Only. Not For Use In Diagnostic Procedures.
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