Render Target: STATIC
Render Timestamp: 2024-12-20T11:18:05.703Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 21:19:08.208
Product last modified at: 2024-12-12T22:30:10.625Z
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PDP - Template Name: Cellular Dyes
PDP - Template ID: *******0000253

MitoTracker® Green FM #9074

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    Product Information

    Product Usage Information

    Each vial of MitoTracker® Green FM contains 50 µg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 74.4 µl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results are obtained by diluting the stock solution directly into normal growth media at a concentration between 100-400 nM and incubating between 15-30 min at 37ºC. After incubation, the cells must be imaged live; DO NOT FIX!

    To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging.

    Storage

    Store lyophilized solid at -20°C dessicated and protected from light. In lyophilized form, this reagent is stable for 12 months. Once reconstituted in DMSO, the solution should be stored at -20°C, protected from light, and must be used within 2 weeks. Avoid freeze-thaw cycles.

    Product Description

    MitoTracker® Green FM is recommended for live cell imaging only; fixation with aldehydes or alcohols will inhibit staining. Excitation: 490 nm, Emission: 516 nm, Molecular Weight: 671.88 g/mol

    Background

    MitoTracker® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker® probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.
      For Research Use Only. Not For Use In Diagnostic Procedures.
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